منابع مشابه
DNA recombination during PCR.
PCR co-amplification of two distinct HIV1 tat gene sequences lead to the formation of recombinant DNA molecules. The frequency of such recombinants, up to 5.4% of all amplified molecules, could be decreased 2.7 fold by a 6 fold increase in Taq DNA polymerase elongation time. Crossover sites mapped essentially to three discrete regions suggesting specific Taq DNA polymerase pause or termination ...
متن کاملDNA Recombination by XPCR
The first step of the Adleman-Lipton extract model in DNA computing is the combinatorial generation of libraries. In this paper a new method is proposed for generating a initial pool, it is a quaternary recombination of strings via application of null context splicing rules. Its implementation, based on a kind of PCR called XPCR, results to be convenient with respect to the standard methods, in...
متن کاملDNA Replication and Recombination
This book represents the proceedings of a Satellite Symposium to the 30th Congress of the International Union of Physiological Sciences, held in Canada during July of 1986. It is the twenty-fourth volume of the neurology and neurobiology collection. The contents of the book are divided into seven sections, beginning with an overview of the field of excitatory amino acid transmission, and a disc...
متن کاملGenetic recombination as DNA repair
Maintenance of sexual reproduction and genetic recombination imposes physiological costs when compared to parthenogenic reproduction, most prominently: for maintaining the corresponding (molecular) machinery, for finding a mating partner, and through the decreased fraction of females in a population, which decreases the reproductive capacity. Based on principles from information theory, we have...
متن کاملDNA Detection Using Recombination Proteins
DNA amplification is essential to most nucleic acid testing strategies, but established techniques require sophisticated equipment or complex experimental procedures, and their uptake outside specialised laboratories has been limited. Our novel approach, recombinase polymerase amplification (RPA), couples isothermal recombinase-driven primer targeting of template material with strand-displaceme...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2003
ISSN: 0021-9258
DOI: 10.1074/jbc.m308228200